User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/10/19

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Sample Preparation

BSA Fluorescence Solution

  1. pH 8
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 63.1 uM
  2. [Au]= 2.39 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 148.9 uL BSA
  2. 255.6 uL Au
  3. 2565.4 uL HOH
  4. 3 uL NaOH

(same as yesterday; same stock solns).

Data Analysis and Results

FLUOR Anneliese Faustino was here 161019 PART 1.PNG

The chart above shows the wavelength (nm) of the emission maximum over time for 0.25 mM Au and 3.125 uM BSA at pH 8. Here we see a plateau around 310 nm until about 3000 seconds, where the max wavelength jumps to about 420 nm and plateaus for the rest of the run time.

FLUOR Anneliese Faustino was here 161019 PART 2.PNG

Here the chart displays the integrated emission intensity over time for 0.25 mM Au and 3.125 uM BSA at pH 8. At 0 seconds, the integrated emission intensity is about 2000 units and increases until a peak around ~5200 units at about 1800s. The emission intensity slowly decreases until about 4000 seconds (4500 intensity units), then slowly increases to about 5000 intensity units for the remainder of the run time.

FLUOR Anneliese Faustino was here 161019 PART 3.PNG

FLUOR Anneliese Faustino was here 161019 PART 4.PNG

FLUOR Anneliese Faustino was here 161019 PART 5.PNG

The above three charts show the emission intensity of the ocean optics scans over time. As we can see, the integrated intensity increases dramatically until about 1000s, where the increase dramatically slows and eventually plateaus out.