User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/14

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Sample Solution for Ocean Optics 160914

  1. Stocks
    1. 4.57 mM Au
    2. 53.3 mM BSA
    3. 0.001 mM HCl
    4. DI Water
  1. Volumes Added to Generate Sample
    1. 175.89 uL BSA
    2. 164.11 uL Au
    3. 30 uL HCl
    4. 2629.99 uL HOH


Ocean Optics Ph 5 part 1 160928.PNG

In the chart above, we see that the maximum absorbance increases over time.

Ocean optics ph 5 part 2 160928.PNG

In the chart above, we see a flat maximum wavelength at about 445 nm until 2800s, where we see a peak and then an increase in maximum wavelength at about 3200 s. The wavelength then plateaus at about 335 nm.

Ocean optics ph 5 part 3 160928.PNG

This chart is similar to the maximum absorbance over time chart as expected.

Comparison of Ocean Optics and Fluorescence for BSA pH4

Fluorescence vs ocean optics ph4.PNG

The integrated intensity versus time (fluorescence) and the absorbance versus time (Ocean Optics) for BSA pH 4 with 0.25 mM and 3.125 mM BSA were plotted in the same chart. There is a dip in the absorbance (Ocean Optics) as the nanoparticles begin forming (peak fluorescence).