User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/20
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BSA Fluorescence Solution
Stock Concentrations 160920
Volumes for making the solution:
The sample was put in the fluorimeter and measurements were taken every five minutes for three hours.
The chart above shows the emission maximums over time for the solution of pH 5 BSA with gold nanoparticles. A major increase in maximum emission occurs around ~7 minutes and another smaller increase occurs around ~17 minutes. The emission maximum then plateaus around 420-440 nm.
The chart above shows that the integrated fluorescence increases rapidly from ~4000-7500 nm in the first 20 min. The integrated fluorescence then continues to increase more slowly from ~7500-8500 for the next 20 min before reaching an asymptote around ~9000 nm for the rest of the experiment.
UV-Vis Solutions for tomorrow
Solutions were put in the oven at 80'C and held for four hours.
Fluorescence vs. Ocean Optics pH 5