User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/13

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BSA UV-Vis 160913 Samples did not form nanoparticles correctly here. Samples were remade as described here. Samples were put into the fluorimeter and analyzed. Analysis is below. Photos of the samples before fluorescence analysis are shown below: This image shows 0.125 mM Fructose, 0.25 mM Au samples with pH 4-12. This image shows 0.25 mM Fructose, 0.25 mM Au samples with pH 4-12. Stock Solutions for 160913 AuCl3 mass = 34.62 mg 25.0 mL volumetric flask MW = 303.3 g/mol concentration = 4.57 mM Bovine Serum Albumin mass = 35.62 mg 10.0 mL volumetric flask MW = 66776 g/mol concentration = 53.3 mM Lysozyme 33.32 mg of lysozyme 10.0 mL water in volumetric flask MW = 14307 g/mol concentration = 232.9 μM Myoglobin 32.23 mg of myoglobin water to 10.0 mL in volumetric flask MW = 16951 g/mol concentration = 137 μM Data 160913 The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 0.125 mM. The chart below shows the absorbance scans across 200-800 nm for pH 4-12 with fructose concentration 0.25 mM. The chart below shows the A(530) for pH 4-12 with fructose concentration 0.125 mM. The chart below shows the A(530) for pH 4-12 with fructose concentration 0.25 mM. Results 160913 Similarly to the last class, the pH 11-12 solutions were more clear than pH 4-10. They still had much lower absorbances. The pH 4-10 samples had similar peak absorbances across the fluorescence spectrum scan (200-800 nm). The A(530) of pH 4-10 samples was also found to be similar across all samples. Calculations for tomorrow 160914 Stocks 4.57 mM Au 53.3 mM BSA 0.001 mM HCl DI Water Volumes Added to Generate Sample 175.89 uL BSA 164.11 uL Au 30 uL HCl 2629.99 uL HOH