User:Allison K. Alix/Notebook/CHEM-581/2013/03/18

Preparation of Liposome

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Objective Prepare Lipid Bilayer Procedure 1) Dissolve 100mg transfection reagent in 5mL chloroform 2) Remove 1mL of solution and place in a seperate vial. Allow to evaporate. 3) Once evaporated, add 1mL phosphate buffer to redissolve lipids 4) 3 freeze-thaw-vortex cycles were applied to the solution to create the multilameller vesicles 5) These multilamellar vesicles were then converted to unilamellar vesicles by passing through a nucleopore membrane indicators methyl violet 0-2 methyl red- 4.4-6.2 bromocresol purple- 5.2-6.8 bromophenol blue 6.0-7.6 cresol red 7.2-8.8 phenolphthalein- 8.3-10.0 Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.