User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/11

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MATA2 PCR on S. cerevisiae

EX Takara RXN Mixture conc 1X 6
Milli Q water 6.15 36.9
Takara EX Buffer 10X 1 6
dNTP 2.5uM 0.8 4.8
Primer Mix 5uM 1 6
EX Taq 5U 0.05 0.3
Template Chelex Extraction 1
54
9.0ul / RXN and 1.0ul Chelex Extraction
Genus species NCYC # sample
Saccharomyces cerevisiae 60893 18
Saccharomyces cerevisiae 60894 19
Saccharomyces cerevisiae 60895 20
Saccharomyces cerevisiae 60896 21
Saccharomyces cerevisiae 1001 (+)
Negative Control (-)
PCR cycle
98C 10 sec
60C 1 min
x15

11Aug2010.jpg