User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/11

Alien genes

MATA2 PCR on S. cerevisiae

EX Takara RXN Mixture	conc	1X	6
Milli Q water		6.15	36.9
Takara EX Buffer	10X	1	6
dNTP	2.5uM	0.8	4.8
Primer Mix	5uM	1	6
EX Taq	5U	0.05	0.3
Template	Chelex Extraction	1
			54
			9.0ul / RXN and 1.0ul Chelex Extraction

Genus	species	NCYC #	sample
Saccharomyces	cerevisiae	60893	18
Saccharomyces	cerevisiae	60894	19
Saccharomyces	cerevisiae	60895	20
Saccharomyces	cerevisiae	60896	21
Saccharomyces	cerevisiae	1001	(+)
Negative Control			(-)

PCR cycle
98C	10 sec
60C	1 min
x15