User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/13

Alien genes

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PCR Clean Up and Cycle Seq S. cerevisiae CS Reaction mixture conc. 1x 4 Big Dye Terminator 0.5 2 DMSO 0.1 0.4 5X Seq Buffer 5X 0.4 0.8 Primer (FWD or RVS) 5uM 0.4 1.6 Template * ~1-3ng/ul 4.6 2.0ul / RXN *QIAquick Cleaned PCR product Cycle Seq Program 96C 10 sec 50C 5 sec 60C 4 min x60 MATA2 FWD and RVS Genus species NCYC # sample Saccharomyces cerevisiae 60893 18 Saccharomyces cerevisiae 60894 19 Saccharomyces cerevisiae 60895 20 Saccharomyces cerevisiae 60896 21