User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/08/13

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PCR Clean Up and Cycle Seq S. cerevisiae

CS Reaction mixture conc. 1x 4
Big Dye Terminator 0.5 2
DMSO 0.1 0.4
5X Seq Buffer 5X 0.4 0.8
Primer (FWD or RVS) 5uM 0.4 1.6
Template * ~1-3ng/ul 4.6
2.0ul / RXN
*QIAquick Cleaned PCR product
Cycle Seq Program
96C 10 sec
50C 5 sec
60C 4 min
x60
MATA2 FWD and RVS
Genus species NCYC # sample
Saccharomyces cerevisiae 60893 18
Saccharomyces cerevisiae 60894 19
Saccharomyces cerevisiae 60895 20
Saccharomyces cerevisiae 60896 21