User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/24

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Alien Genes New Primer Screen

Reaction mixture conc. 1x x6.5 x8.5 x2 ' ' '
Temp (39 - 41) ?? 2 13 17
TopTaq Buffer 10x 1 6.5 8.5
dNTP mix 10 mM each 0.2 1.3 1.7
Primer mix 5 uM each 1
TopTaq 5 U/ul 0.05 0.325 0.425
Milli Q 5.75 37.375 48.875
10 ul
No. Primer mix No. Primer mix
PCR cycle (2 STEP) TaKaRa Dice 1 AV10045 1 AV10134
94C 3 min 2 AV10044 2 AV10121
3 AV10043 3 AV10084
98C 10 sec 4 AV10042 4 AV10045
60C 1 min 5 AV10041 5 AV10044
x35 6 AV10034 6 AV10034
7 AV10027
72C 5 min No. Sample 8 AV10025
39 Tsukuba 3D4
No. Sample
40 Woods Hole 1A6
41 Woods Hole 1C2

Loaded 2.0 ul of each sample

0041.jpg

Loaded 8.0 ul of each sample

0043.jpg


Reaction mixture conc. 1x x97 ' No. Primer mix
Temp (49 - 60) ?? 2 194 1 AV10134
TopTaq Buffer 10x 1 97 2 AV10121
dNTP mix 10 mM each 0.2 19.4 3 AV10084
Primer mix 5 uM each 1 4 AV10045
TopTaq 5 U/ul 0.05 4.85 5 AV10044
Milli Q 5.75 557.75 6 AV10034
10 ul 7 AV10027
8 AV10025
PCR cycle
94C 3 min No.
1 Pennsylvania (PA) D4
98C 10 sec 2 Tsukuba 1 B5
60C 3 min 3 Yokohama A2
x35 4 Baltimore C1
5 Ithaca 1 A4
72C 5 min 6 Pennsylvania (PA) B6
7 Yokohama B2
8 Yokohama A4
9 Ithaca 1 C4
10 Ithaca 1 D5
11 Baltimore C5
12 E. Windsor A2

Loaded 2.0ul of each sample

0045-1.jpg