User:Alexander S Mikheyev/Notebook/rotifer alien genes/2010/03/24

Alien genes

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Alien Genes New Primer Screen Reaction mixture conc. 1x x6.5 x8.5 x2 ' ' ' Temp (39 - 41) ?? 2 13 17 TopTaq Buffer 10x 1 6.5 8.5 dNTP mix 10 mM each 0.2 1.3 1.7 Primer mix 5 uM each 1 TopTaq 5 U/ul 0.05 0.325 0.425 Milli Q 5.75 37.375 48.875 10 ul No. Primer mix No. Primer mix PCR cycle (2 STEP) TaKaRa Dice 1 AV10045 1 AV10134 94C 3 min 2 AV10044 2 AV10121 3 AV10043 3 AV10084 98C 10 sec 4 AV10042 4 AV10045 60C 1 min 5 AV10041 5 AV10044 x35 6 AV10034 6 AV10034 7 AV10027 72C 5 min No. Sample 8 AV10025 39 Tsukuba 3D4 No. Sample 40 Woods Hole 1A6 41 Woods Hole 1C2 Loaded 2.0 ul of each sample Loaded 8.0 ul of each sample Reaction mixture conc. 1x x97 ' No. Primer mix Temp (49 - 60) ?? 2 194 1 AV10134 TopTaq Buffer 10x 1 97 2 AV10121 dNTP mix 10 mM each 0.2 19.4 3 AV10084 Primer mix 5 uM each 1 4 AV10045 TopTaq 5 U/ul 0.05 4.85 5 AV10044 Milli Q 5.75 557.75 6 AV10034 10 ul 7 AV10027 8 AV10025 PCR cycle 94C 3 min No. 1 Pennsylvania (PA) D4 98C 10 sec 2 Tsukuba 1 B5 60C 3 min 3 Yokohama A2 x35 4 Baltimore C1 5 Ithaca 1 A4 72C 5 min 6 Pennsylvania (PA) B6 7 Yokohama B2 8 Yokohama A4 9 Ithaca 1 C4 10 Ithaca 1 D5 11 Baltimore C5 12 E. Windsor A2 Loaded 2.0ul of each sample