| Plate
|
Well
|
Genus
|
12/2/2009 Count
|
Sample
|
Extract
|
'
|
Reagents
|
Concentration
|
1X
|
5X
|
| AV 10-06-09 |
well 6 |
Adineta |
100+ |
2 |
|
|
H20 |
|
4.75 |
23.75
|
| WH1 |
B3 |
Adineta |
20+ |
7 |
use 12/7 |
|
10X Buffer |
10X |
1 |
5
|
| WH1 |
B5 |
Adineta |
20+ |
8 |
use 12/7 |
|
dye |
10X |
1 |
5
|
| WH1 |
B6 |
Adineta |
50+ |
9 |
use 12/7 |
|
dNTP |
10mM |
0.2 |
1
|
|
|
|
|
|
|
|
primer mix |
5uM |
1 |
5
|
|
|
|
|
|
|
|
Taq |
5U/uM |
0.05 |
0.25
|
| EXTRACTION |
|
|
|
|
|
|
DNA |
variable |
2 |
|
| InstaGene Matrix |
200ul per sample |
|
|
|
|
|
TOTAL |
|
10 |
40
|
| 56C |
30 min |
|
|
|
|
|
|
|
Cocktail per well = 8.0ul |
|
| 99.9C |
8min |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
On Gels |
|
| 12/2/2009 |
5-10 individuals |
12/7/2009 |
10-20 individuals |
|
|
|
Primers |
AV10158 |
#1-4 |
|
|
|
|
|
|
|
|
|
AV10153 |
#5-8 |
|
|
|
|
|
|
|
|
|
AV10151 |
#9-12 |
|
| load 2.0ul 2-log ladder and 2.0ul sample 1% gel |
|
|
|
|
|
|
|
AV10150 |
#13-16 |
|
| Sample #2 Positive Control |
|
|
|
|
|
|
|
AV10140 |
#17-20 |
|
|
|
|
|
|
|
|
|
AV10134 |
#21-24 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PCR |
95C |
3 min |
|
|
|
|
|
|
|
|
|
95C |
10 sec |
|
|
|
|
|
|
|
|
|
60C |
2 min |
|
|
|
|
|
|
|
|
|
35 cycles |
|
|
|
|
Alien genes
Main project page
Previous entry Next entry
