User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/15

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Sample 7,8&9 ReAmp with Primers 1-6

Plate Well Genus 12/2/2009 Count Sample Extract ' Reagents Concentration 1X 5X
AV 10-06-09 well 6 Adineta 100+ 2 H20 4.75 23.75
WH1 B3 Adineta 20+ 7 use 12/7 10X Buffer 10X 1 5
WH1 B5 Adineta 20+ 8 use 12/7 dye 10X 1 5
WH1 B6 Adineta 50+ 9 use 12/7 dNTP 10mM 0.2 1
primer mix 5uM 1 5
Taq 5U/uM 0.05 0.25
EXTRACTION DNA variable 2
InstaGene Matrix 200ul per sample TOTAL 10 40
56C 30 min Cocktail per well = 8.0ul
99.9C 8min
On Gels
12/2/2009 5-10 individuals 12/7/2009 10-20 individuals Primers AV10158 #1-4
AV10153 #5-8
AV10151 #9-12
load 2.0ul 2-log ladder and 2.0ul sample 1% gel AV10150 #13-16
Sample #2 Positive Control AV10140 #17-20
AV10134 #21-24
PCR 95C 3 min
95C 10 sec
60C 2 min
35 cycles
15dec09 sample 7 8 9 redos text.jpg