User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/17

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PEG precipitation

  • recipe:
    • 3g PEG 8000
    • 2.19g NaCl
    • Bring up to 15ml with good water.
  • added 8ul PEG to PCR
  • spun at max speed for 15 min
  • removed PEG, added 150ul cold 80% ethanol
  • spin 7 min
  • remove ethanol, dry
  • re-suspend in 10 ul water
  • nanodrop
Sample ID Nucleic Acid Conc. Unit 260/280 260/230
AV10153_Boston_A1 40.1 ng/µl 1.9 1.21
AV10153_WH1_C4 19.5 ng/µl 2.16 0.65
AV10150_CC_A3 41.8 ng/µl 2.16 1.21
AV10151_CC_A3 47.7 ng/µl 1.96 1.16
AV10140_CC_A3 46.7 ng/µl 1.98 1.08