User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/03

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Adineta Amp Primers 1-6

Plate	Well	Genus	12/2/2009 Count	Sample	'	Reagents	Concentration	1X	15X
Adenita 23Nov09	B3	Adineta	30+	1		H20		4.75	71.25
AV 10-06-09	well 6	Adineta	100+	2		10X Buffer	10X	1	15
Boston	A1	Adineta	10+	3		dye	10X	1	15
Boston	A4	Adineta	50+	4		dNTP	10mM	0.2	3
CC	A1	Adineta	subs 100+	5		primer mix	5uM	1	15
CC	A3	Adineta	subs 100+	6		Taq	5U/uM	0.05	0.75
WH1	B3	Adineta	20+	7		DNA	variable	2
WH1	B5	Adineta	20+	8		TOTAL		10	120
WH1	B6	Adineta	50+	9				Cocktail per well = 8.0ul
WH1	C4	Adineta	30+	10
WH2	A6	Adineta	10+	11				On Gels
WH3	A5	Adineta	20+	12		Primers	AV10158	#1-13
WH3	C2	Adineta	7	13			AV10153	#14-26
							AV10151	#27-39
EXTRACTION							AV10150	#40-52
5-10 individuals							AV10140	#53-65
							AV10134	#66-78
InstaGene Matrix	200ul per sample
56C	30 min					PCR	95C	3 min
99.9C	8min						95C	10 sec
							60C	2 min
							35 cycles

load 2.0ul 1kb ladder and 2.0ul sample 1% gel

• summary (sample 2 should be a positive control):

locus	extract
AV10158	2
AV10153	2
AV10153	6
AV10153	10
AV10151	2
AV10151	6
AV10150	2
AV10150	6
AV10140	2
AV10140	6
AV10134	2
AV10134	5