User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/03

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Alien genes Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Adineta Amp Primers 1-6

Plate Well Genus 12/2/2009 Count Sample ' Reagents Concentration 1X 15X
Adenita 23Nov09 B3 Adineta 30+ 1 H20 4.75 71.25
AV 10-06-09 well 6 Adineta 100+ 2 10X Buffer 10X 1 15
Boston A1 Adineta 10+ 3 dye 10X 1 15
Boston A4 Adineta 50+ 4 dNTP 10mM 0.2 3
CC A1 Adineta subs 100+ 5 primer mix 5uM 1 15
CC A3 Adineta subs 100+ 6 Taq 5U/uM 0.05 0.75
WH1 B3 Adineta 20+ 7 DNA variable 2
WH1 B5 Adineta 20+ 8 TOTAL 10 120
WH1 B6 Adineta 50+ 9 Cocktail per well = 8.0ul
WH1 C4 Adineta 30+ 10
WH2 A6 Adineta 10+ 11 On Gels
WH3 A5 Adineta 20+ 12 Primers AV10158 #1-13
WH3 C2 Adineta 7 13 AV10153 #14-26
AV10151 #27-39
EXTRACTION AV10150 #40-52
5-10 individuals AV10140 #53-65
AV10134 #66-78
InstaGene Matrix 200ul per sample
56C 30 min PCR 95C 3 min
99.9C 8min 95C 10 sec
60C 2 min
35 cycles
load 2.0ul 1kb ladder and 2.0ul sample 1% gel
03dec09 Gel 1 text.jpg
03dec09 Gel 2 text.jpg
03dec09 Gel 3 text.jpg
  • summary (sample 2 should be a positive control):
locus extract
AV10158 2
AV10153 2
AV10153 6
AV10153 10
AV10151 2
AV10151 6
AV10150 2
AV10150 6
AV10140 2
AV10140 6
AV10134 2
AV10134 5