User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/01

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Test Redesigned Primers

PCR


Reagents Concentration 1X 5X
H20 5.75 28.75
10X Buffer 10X 1 5
dye 10X 1 5
dNTP 10mM 0.2 1
Primers 5uM 1 **
Taq 5U/uM 0.05 0.25
*DNA 20ng/ul 1 5
TOTAL 10 45
Cocktail per well = 9.0ul
* DNA Adenita vaga ** Primers AV10126 F2+R2
Adenita ricciae AV10094 F2+R2
HIS F1+R1
HIS F1+R2
PCR 95C 3 min
95C 10 sec
60C 2 min
35 cycles
Gid 2009-12-01 16hr 42min.jpg
' vaga ricciae vaga ricciae vaga ricciae vaga ricciae '
1Kb Ladder well 1 well 2 well 3 well 4 well 5 well 6 well 7 well 8 1Kb Ladder
AV10126 AV10126 AV10094 AV10094 HISF1+R1 HISF1+R1 HISF1+R2 HISF1+R2
load 2.0ul ladder and 3.0ul sample on 1%gel