Talk:CH391L/S12/Protein regulators of transcription

From OpenWetWare
Jump to navigationJump to search

  • Brian Renda 17:23, 5 March 2012 (EST):to add: gal, tet on, off, copper, m-toluic acid
  • Jeffrey E. Barrick 12:18, 7 March 2012 (EST):There is one distinction between Lac and Ara regulation that is important. Then the regulatory protein is absent in the Lac system (for example, missing from a strain), your promoter is constitutively ON. Then the regulatory protein from the Ara system is absent, your promoter is constitutively OFF. These states will also result if a mutation happens that knocks out the regulatory protein, which can make the systems differentially robust to mutations.
  • Yi Kou 12:42, 19 March 2012 (EDT): sorry for adding this late, are there any studies on leaky expression of lac and ara operons? I am kind of interested in how they quantify this effect.