Streptomyces:Media and Solutions/Antibiotic & Resistance Marker Information

Media and Solutions - Antibiotic & Resistance Marker Information

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Name	Stock Concentration (mg/ml)	Final Concentration (μg/ml)	Usage in 10mL (μL)	Dissolve in
Ampicillin	100	100	10	sdH₂0
Apramycin	100	50	5	sdH₂0
Chloramphenicol	25	25	10	50%-100% EtOH
Hygromycin	50	25	5	sdH₂0
Kanamycin	100	50	5	sdH₂0
Nalidixic Acid	25	25	10	0.2M NaOH
Thiostrepton(e)	50	50	10	100% DMSO or 1:1 DMSO:EtOH
Viomycin	30	30	10	sdH₂0

E.coli Strain	Plasmid / Cosmid	E.coli Resistance Marker	Plasmid Resistance Marker	Temperature Sensitive Replicon (ts)
BW25113	pIJ790 (λRED)	None	cat	Replication @ 30C, Loss of λRED @ 37C
DH5α	None	N/A
DH5α	BT340 (FLP Recombinase)	None	cat / blacarb	Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C
ET12567 [dam-13::Tn9]	pUZ8002	cat	neo
	SuperCos 1		neo / blacarb
	pIJ773		acc(3)IV