Streptomyces:Media and Solutions

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Media and Solutions

Streptomyces @ UEA

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Media and Solutions

Soya Flour Mannitol Agar (Also known as MS or SFM)

Add 2 grams of soya flour and 2 grams of agar to 10 x 250 ml conical flasks. Dissolve 20 grams mannitol in 1 litre of tap water. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice.

Oatmeal agar (for S. scabies)

Add 4 grams of rolled oats and 2 grams of agar to 10 x 250 ml conical flasks. Add 100 ml tap water to each of the 10 flasks, add bungs and foil and autoclave twice.

Instant Potatomash Medium (for S. scabies)

Add 2 grams of agar to 10 x 250 ml conical flasks. Boil 1 litre of tap water and dissolve in 50 grams of Smash powder. Dispense 100 ml into each of the 10 flasks, add bungs and foil and autoclave twice.