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CHEM-ENG590E Wiki Textbook:Microcontact Printing: Difference between revisions
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CHEM-ENG590E Wiki Textbook:Microcontact Printing (view source)
Revision as of 09:51, 15 April 2016
, 15 April 2016→Applications
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[[Image:F5.medium.gif|right|thumbnail|'''Figure 3.''' Schematic of method for generating micropatterned cocultures, from Bhatia et al.<sup>[5]</sup> (A) Borosilicate substrates were coated with photoresist (a UV-sensitive polymer) and exposed to light through a mask, creating a photoresist pattern. (B) Photoresist was visualized. (C) Collagen I was immobilized, followed by removal of the photoresist, which yielded a collagen-glass pattern. (D) Indirect immunofluorescence verified collagen immobilization. (E) Patterned substrates were exposed to hepatocytes, resulting in micropatterned hepatocytes. (F) 200 μM lanes of hepatocytes with 500 μM lane spacing. (G) Addition of fibroblasts to create micropatterned cocultures. (H) 2 distinct cell types in micropatterned coculture are clear.]] | [[Image:F5.medium.gif|right|thumbnail|'''Figure 3.''' Schematic of method for generating micropatterned cocultures, from Bhatia et al.<sup>[5]</sup> (A) Borosilicate substrates were coated with photoresist (a UV-sensitive polymer) and exposed to light through a mask, creating a photoresist pattern. (B) Photoresist was visualized. (C) Collagen I was immobilized, followed by removal of the photoresist, which yielded a collagen-glass pattern. (D) Indirect immunofluorescence verified collagen immobilization. (E) Patterned substrates were exposed to hepatocytes, resulting in micropatterned hepatocytes. (F) 200 μM lanes of hepatocytes with 500 μM lane spacing. (G) Addition of fibroblasts to create micropatterned cocultures. (H) 2 distinct cell types in micropatterned coculture are clear.]] | ||
Cell-cell interactions may be studied using micropatterning techniques like printing. Bhatia et al. demonstrated microcontact printing allowed spatial control over liver cell populations, and thus | Cell-cell interactions may be studied using micropatterning techniques like printing. Bhatia et al. demonstrated that microcontact printing allowed spatial control over liver cell populations, and thus allowed a highly sophisticated method of investigation of cell growth, movement, shape, and death. In this study, collagen structures were immobilized under a patterned photoresist mask. The collagen then acted as an adhesive extracellular matrix for a patterning of hepatocytes and nonparenchymal cells. The cell interactions in this microenvironment were then studied. | ||
'''Biosensors<sup>[11]</sup>''' | '''Biosensors<sup>[11]</sup>''' | ||
