1,304
edits
20.109(F15):Homework: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Due M2D2
No edit summary |
|||
| Line 89: | Line 89: | ||
#Toward the end of class, we will discuss an article from the primary literature. Specifically, we will discuss the construction and analysis of the inverse pericam (IPC) multi-component calcium sensor. To prepare for this discussion, you should do a close reading of the paper by [http://www.ncbi.nlm.nih.gov/pubmed/11248055 Nagai et al] that introduced IPC. For context, you are also encouraged to read the short paper by [http://www.ncbi.nlm.nih.gov/pubmed/7809066?ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Heim, Prasher, and Tsien], in which the very first attempt to mutagenize GFP is described, and which is a fine introduction to some of the concepts and methods used in this module. Though you should read the entire paper, be prepared to specifically discuss the section assigned to your group on the [http://openwetware.org/wiki/20.109%28F15%29:Prepare_expression_system_%28Day2%29#Part_5:_Journal_article_discussion M2D2 page]. | #Toward the end of class, we will discuss an article from the primary literature. Specifically, we will discuss the construction and analysis of the inverse pericam (IPC) multi-component calcium sensor. To prepare for this discussion, you should do a close reading of the paper by [http://www.ncbi.nlm.nih.gov/pubmed/11248055 Nagai et al] that introduced IPC. For context, you are also encouraged to read the short paper by [http://www.ncbi.nlm.nih.gov/pubmed/7809066?ordinalpos=9&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Heim, Prasher, and Tsien], in which the very first attempt to mutagenize GFP is described, and which is a fine introduction to some of the concepts and methods used in this module. Though you should read the entire paper, be prepared to specifically discuss the section assigned to your group on the [http://openwetware.org/wiki/20.109%28F15%29:Prepare_expression_system_%28Day2%29#Part_5:_Journal_article_discussion M2D2 page]. | ||
#Create a diagram that depicts the aspects of your plasmid, which contains your mutated inverse pericam. Be sure to highlight the following: | |||
#*pericam protein coding sequence | |||
#*primer site | |||
#*location of both mutations | |||
#*location of the introduced restriction enzyme recognition sequence | |||
#*'''Note:''' Use the diagrams below as a guide. | |||
#The following assignment will prepare you to set up your digestion reactions. On the whole, Day 2 will likely run long, so reading ahead and completing as many calculations as possible in advance will be of help in making the day run smoothly. | #The following assignment will prepare you to set up your digestion reactions. On the whole, Day 2 will likely run long, so reading ahead and completing as many calculations as possible in advance will be of help in making the day run smoothly. | ||
