742
edits
DNA ligation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
no edit summary
No edit summary |
|||
| Line 66: | Line 66: | ||
Ligation efficiency was '''severely decreased''' (x10000) by | Ligation efficiency was '''severely decreased''' (x10000) by | ||
#Using DNA fragments that have been exposed to ethidium bromide and UV during the gel extraction procedure (''difficult to avoid but heartily recommended'') | #Using DNA fragments that have been exposed to ethidium bromide and UV during the gel extraction procedure (''difficult to avoid but heartily recommended'') | ||
#Using the NEB Quick Ligation Kit | #Using the NEB Quick Ligation Kit (''possibly a bad batch'') | ||
==Notes== | ==Notes== | ||
