2,695
edits
Lidstrom:Buffers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→General Info
| Line 5: | Line 5: | ||
== General Info == | == General Info == | ||
pH = pK<sub>a</sub> + log[A<sup>-</sup>]/[HA] | * Henderson-Hasselbalch equation: pH = pK<sub>a</sub> + log[A<sup>-</sup>]/[HA] | ||
** '''Note: this equation is only an approximation!''' In reality, pH depends on the dilution of a buffer, and the ionic strength of the liquid! See below. | |||
Buffers are usually used within pK ± 1. | Buffers are usually used within pK ± 1. | ||
