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Lidstrom:Buffers: Difference between revisions
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→How does temperature affect the pH of a buffer?
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* [http://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf Applichem publication] | * [http://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf Applichem publication] | ||
** The pH value of a Tris solution set to a pH of 7.8 at room temperature is 8.4 at 0°C and 7.4 at 37°C. | ** The pH value of a Tris solution set to a pH of 7.8 at room temperature is 8.4 at 0°C and 7.4 at 37°C. | ||
=== How do the presence of salts like NaCl affect the pH of a buffer? === | |||
* The presence of other salts affects the ionic strength of the buffer, and often change the measured pH value by several tenths of a point. See discussion of phosphates below for a specific example. | |||
=== What concentration should my buffer be for an enzyme assay?=== | === What concentration should my buffer be for an enzyme assay?=== | ||
