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Lidstrom:Buffers: Difference between revisions
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→Notes specific to buffers
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*pH rane: 5.8-8 | *pH rane: 5.8-8 | ||
* Phosphate buffering is based on the fact that phosphate can be in four states based on the pH. | * Phosphate buffering is based on the fact that phosphate can be in four states based on the pH. | ||
** [[image:2014_02_09 phosphate states.png]] | ** [[image:2014_02_09 phosphate states.png|thumb|center|the pH dependent forms of phosphate ([http://en.wikipedia.org/wiki/Phosphate wikipedia])]] | ||
** [[image:2014_02_09 phosphate state at various pHs.png]] | ** [[image:2014_02_09 phosphate state at various pHs.png]] | ||
** It will shift between the different levels of protonation based on how many protons are available in solution. Thus it can be a source/sink for protons. Like all (good) buffers, if more H+ ions show up in solution, it can help absorb these and maintain the initial pH. Converseley, it can release H+ ions when they disappear from solution. | ** It will shift between the different levels of protonation based on how many protons are available in solution. Thus it can be a source/sink for protons. Like all (good) buffers, if more H+ ions show up in solution, it can help absorb these and maintain the initial pH. Converseley, it can release H+ ions when they disappear from solution. | ||
