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Lidstrom:Buffers: Difference between revisions
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** The pH value of a Tris solution set to a pH of 7.8 at room temperature is 8.4 at 0°C and 7.4 at 37°C. | ** The pH value of a Tris solution set to a pH of 7.8 at room temperature is 8.4 at 0°C and 7.4 at 37°C. | ||
=== What concentration should my buffer be for an enzyme assay?=== | |||
* cross-reference: [[Lidstrom:Enzyme_Assay_Basics#Buffer]] | |||
* ""An adequate buffer capacity is often only reached at concentrations higher than 25mM. However, hiher buffer concentrations and related ionic strengths can inhibit enzyme activity.. Suitable initial concentrations are therefore between 10 and 25mM. If, after addition of the protein or enzyme, the pH value changes by more than 0.05 units, the concentration of the buffer can first be increased to 50mM. Up to this concentration, no interference was observed with the Good buffers in cell culture experiments." --[http://www.applichem.com/fileadmin/Broschueren/BioBuffer.pdf Applichem booklet] | |||
=== How do method developer chose between sodium and potassium phospahte buffers? === | === How do method developer chose between sodium and potassium phospahte buffers? === | ||
* Facts: | * Facts: | ||
