Lidstrom:Buffers: Difference between revisions

Lidstrom:Buffers (view source)

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 === How does temperature affect the pH of a buffer? ===
-Amine-containing buffers are most sensitive to changes in temperature.  Example: Tris-HCl adjusted to pH=8.0 at 25°C will have a pH of 8.78 at 0°C, whereas carboxylic acid buffers are least sensitive to changes in temperature.  Example: acetate buffer adjusted to pH 4.5 at 25°C will have a pH of 4.495 at 0°C.  '''These differences are due to the differences in ΔH for ionization of the acids.'''
+Amine-containing buffers are most sensitive to changes in temperature.  Example: Tris-HCl adjusted to pH=8.0 at 25°C will have a pH of 8.78 at 0°C.
+Carboxylic acid buffers are least sensitive to changes in temperature.  Example: acetate buffer adjusted to pH 4.5 at 25°C will have a pH of 4.495 at 0°C.
+'''These differences are due to the differences in ΔH for ionization of the acids.'''
 (source: [http://www.amazon.com/Enzyme-Assays-Practical-Approach-Series/dp/0199631425/ref=sr_1_1?ie=UTF8&qid=1383921457&sr=8-1&keywords=0199631425 ISBN 0-19-963142-5] pg 319)