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Lidstrom:Buffers: Difference between revisions
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== General Info == | == General Info == | ||
pH = pK<sub>a</sub? + log[A<sup>-<sup>]/[HA] | |||
Buffers are usually used within one pK -1 to pK +1. | |||
(source: [http://www.amazon.com/Enzyme-Assays-Practical-Approach-Series/dp/0199631425/ref=sr_1_1?ie=UTF8&qid=1383921457&sr=8-1&keywords=0199631425 ISBN 0-19-963142-5] pg 318) | |||
== Does pH of a buffer depend on the concentration of buffer? == | == Does pH of a buffer depend on the concentration of buffer? == | ||
A buffer would be expected to maintain its pH upon dilution, if both [A-] and [HA] are reduced in equivalent proportions | |||
(source: [http://www.amazon.com/Enzyme-Assays-Practical-Approach-Series/dp/0199631425/ref=sr_1_1?ie=UTF8&qid=1383921457&sr=8-1&keywords=0199631425 ISBN 0-19-963142-5] pg 318) | |||
== Resources== | == Resources== | ||
