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BISC209/F13: Lab3: Difference between revisions
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→SpectraMax 190:
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Close the Spectromax software. DON'T SAVE! (You have already saved and exported adequately.)<BR> | Close the Spectromax software. DON'T SAVE! (You have already saved and exported adequately.)<BR> | ||
Shut off the SpectraMax spectrophotometer unless you know another group is waiting. | Shut off the SpectraMax spectrophotometer unless you know another group is waiting, or unless your instructor tells you to leave it on for the week we collect data. PLease do'''NOT''' shut down the computer!!!<BR> | ||
Eject the Flash drive hardware properly by right clicking on the left image on the bottom of the computer's tool bar. If that doesn't work, go to Computer--USB (D:) Eject<BR> | Eject the Flash drive hardware properly by right clicking on the left image on the bottom of the computer's tool bar. If that doesn't work, go to Computer--USB (D:) Eject<BR> | ||
Remove Flash drive and take it to the lab computer facing the Spectromax and plug it into a USB port. | Remove Flash drive and take it to the lab computer facing the Spectromax and plug it into a USB port. | ||
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'''DATA STORAGE:'''<BR> | '''DATA STORAGE:'''<BR> | ||
Open your team's Data folder from Sakai and download and open your group's Biolog data sheet. The file is called: BIOLOG-CMD_calc&GraphGroup__2013fa. <BR> | Open your team's Data folder from Sakai and download and open your group's Biolog data sheet. The file is called: BIOLOG-CMD_calc&GraphGroup__2013fa. <BR> | ||
Then EDIT: PASTE | Then EDIT: PASTE the data carefully to your team's Excel spreadsheet making SURE that you are on the appropriate day (found in tabs on the bottom of the workbook). Note that each day that you collect data has a separate page in this spreadsheet.<BR> | ||
Copy and paste one column in the spectrophotometer 96 well format at time into your group's spreadsheet. After pasting column 1, 2 and 3 you will begin the next set of replicates (columns 4-6 then 7-9). <BR> | Copy and paste one column in the spectrophotometer 96 well format at time into your group's spreadsheet. After pasting column 1, 2 and 3 you will begin the next set of replicates (columns 4-6 then 7-9). <BR> | ||
'''This is a very important step because it aligns the replicate cells properly. '''<BR> | '''This is a very important step because it aligns the replicate cells properly. '''<BR> | ||
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[[Image: Read2.jpg]] | [[Image: Read2.jpg]] | ||
When you have finished | When you have finished eject the Flashdrive (move the icon to trash on a MAC). Wait for the icon to disappear and please store the flash drive to the USB port in the computer for the Spectromax. If it is not there, send a message to the class to see if someone took it home by mistake and email your instructor. You can still transfer your data if the disk is missing by reading off the data from the Spectromax computer to your open data Spreadsheet. (The 2 computer screens are close enough to allow that.) | ||
PRECAUTION: If you haven’t saved your data and another group reads their plate, your data will be overwritten and lost.<BR><BR> | PRECAUTION: If you haven’t saved your data and another group reads their plate, your data will be overwritten and lost.<BR><BR> | ||
