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BISC209/F13: Lab3: Difference between revisions
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View of back of Spectra Max 190 <BR> | View of back of Spectra Max 190 <BR> | ||
If the Spectra MAX 190 is not on, turn it on using the switch located next to the plug in the back on the right hand side (as you face the spec). The drawer will open. | |||
The drawer may, or may not, close on its own. If it doesn't close, please press the DRAWER button found on the Spectra MAX control panel (pictured below). It is important | The drawer may, or may not, close on its own. If it doesn't close, please press the DRAWER button found on the Spectra MAX control panel (pictured below). It is important | ||
to keep the drawer closed as much as possible to prevent dust from entering the spectrophotometer. | to keep the drawer closed as much as possible to prevent dust from entering the spectrophotometer. | ||
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[[Image: Spectramax190read.jpg]]<BR> | [[Image: Spectramax190read.jpg]]<BR> | ||
The machine will automatically start a required calibration. Allow up to 10 minutes for the Specta MAX 190 to warm up after the calibration and BEFORE you attempt to insert your plate. | The machine will automatically start a required calibration. Allow up to 10 minutes for the Specta MAX 190 to warm up after the calibration and BEFORE you attempt to insert your plate. | ||
The computer should be on sleep. If it doesn't "wake up" when you move the mouse, Press the on/off control on the keyboard ONE time only. If a User Lock screen appears, ignore it. This computer has no password so just hit ENTER (return). | The computer should be on sleep. If it doesn't "wake up" when you move the mouse, Press the on/off control on the keyboard ONE time only. If a User Lock screen appears, ignore it. This computer has no password so just hit ENTER (return). | ||
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Click OK | Click OK | ||
The spec should be ready to read all the wells in a 96 well plate. If the Green Read button on the top toolbar is missing, it means the instrument is Offline. To get it back Online, Click the Spectromax 190 icon on the left of the top toolbar. An Instrument Connect popup window should appear with an hourglass. Be patient, the hourglass takes awhile to go away. Eventually an Available Instrument window will appear. Choose Com3 Spectromax190. Then click OK. The Green Read icon should be visible. | The spec should be ready to read all the wells in a 96 well plate. If the Green Read button on the top toolbar is missing, it means the instrument is Offline. To get it back Online, Click the Spectromax 190 icon [[Image: SpectraMax190specimage.jpg]] on the left of the top toolbar. An Instrument Connect popup window should appear with an hourglass. Be patient, the hourglass takes awhile to go away. Eventually an Available Instrument window will appear. Choose Com3 Spectromax190. Then click OK. The Green Read icon should be visible in the toolbar. | ||
[[Image: CorrectViewspecimage.jpg]] | |||
Click Read. | Click Read. | ||
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The data should be saved automatically in softmax pro, but please also save to our BISC209 folder on the desktop, labeling clearly with your group letter and the date. | The data should be saved automatically in softmax pro, but please also save to our BISC209 folder on the desktop, labeling clearly with your group letter and the date. | ||
Because the instrument computer is not networked, you will have to Save the data to a FLASH DRIVE to export it to your Sakai data file. <BR> | Because the instrument computer is not networked, you will have to Save the data to a FLASH DRIVE to export it to your Sakai data file. <BR> | ||
Export the labeled data to the Flash drive by clicking on the blue image of the plate in the upper left of the screen. From the menu that appears, click Export. Then Choose Plate1 and Output format PLATE raw (.txt). Click OK. Then SAVE AS with your site and date AND change the file type to .xls (not the default!). Save to the USB (D:) drive. | Export the labeled data to the Flash drive by clicking on the blue image [[Image: BluePlatespecimage.jpg]] of the plate in the upper left of the screen. From the menu that appears, click Export. Then Choose Plate1 and Output format PLATE raw (.txt). Click OK. Then SAVE AS with your site and date AND change the file type to .xls (not the default!). Save to the USB (D:) drive. | ||
Close the Spectromax software. DON'T SAVE! (You have already saved and exported adequately.)<BR> | Close the Spectromax software. DON'T SAVE! (You have already saved and exported adequately.)<BR> | ||
