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BISC209/F13: Lab3: Difference between revisions
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→Community EXOENZYME Prevalence:
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Using your P200 micropipet and sterile tips, dispense 100µl of one of the soil extract dilutions into the center of each of two pre-labeled starch agar plate at the appropriate dilutions. Choose the dilution that is expected to result in 30-300 cfu/g soil. Use a sterile, disposable spreader to evenly distribute the extract aliquot. Repeat for a soil extract dilution that is 10 fold more and one 10 fold less dilute. Incubate all plates at RT until mature, visible colonies appear. Move the culture plates to the cold room before they become overgrown.<BR> | Using your P200 micropipet and sterile tips, dispense 100µl of one of the soil extract dilutions into the center of each of two pre-labeled starch agar plate at the appropriate dilutions. Choose the dilution that is expected to result in 30-300 cfu/g soil. Use a sterile, disposable spreader to evenly distribute the extract aliquot. Repeat for a soil extract dilution that is 10 fold more and one 10 fold less dilute. Incubate all plates at RT until mature, visible colonies appear. Move the culture plates to the cold room before they become overgrown.<BR> | ||
3. '''Cellulose Congo Red Agar Medium is used to determine the % of cellulolytic microbes (those producing cellulase and able to digest cellulose) ''' | 3. '''Cellulose Congo Red Agar Medium is used to determine the % of cellulolytic microbes (those producing cellulase and able to digest cellulose) ''' <BR> | ||
Using your P200 micropipet and sterile tips, dispense 100µl of one of the soil extract dilutions into the center of each of the pre-labeled cellulose congo red agar replicate plates. Choose the dilution that is expected to result in 30-300 cfu/g soil. Use a sterile, disposable spreader to evenly distribute the extract aliquot. Repeat for a soil extract dilution that is 10 fold more and one 10 fold less dilute. The ashed, acid-washed cellulose and the Congo red in the medium will allow enumeration of cellulose-digesting bacteria in soil. Incubate all plates at RT until mature, visible colonies appear. Move the culture plates to the cold room before they become overgrown.<BR> | Using your P200 micropipet and sterile tips, dispense 100µl of one of the soil extract dilutions into the center of each of the pre-labeled cellulose congo red agar replicate plates. Choose the dilution that is expected to result in 30-300 cfu/g soil. Use a sterile, disposable spreader to evenly distribute the extract aliquot. Repeat for a soil extract dilution that is 10 fold more and one 10 fold less dilute. The ashed, acid-washed cellulose and the Congo red in the medium will allow enumeration of cellulose-digesting bacteria in soil. Incubate all plates at RT until mature, visible colonies appear. Move the culture plates to the cold room before they become overgrown.<BR> | ||
'''Cellulose Congo Red Agar: '''<BR> | '''Cellulose Congo Red Agar: '''<BR> | ||
