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BISC209/F13: Lab3: Difference between revisions
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(0.3% Beef extract, 0.5% Peptone, 1.5% Agar at pH 6.6- 7.0 at 25°C) is used to determine comparative number of total culturable bacteria:<BR> | (0.3% Beef extract, 0.5% Peptone, 1.5% Agar at pH 6.6- 7.0 at 25°C) is used to determine comparative number of total culturable bacteria:<BR> | ||
Using your P200 micropipet and sterile tips, dispense 100µl of the appropriate soil extract dilution onto each of two pre-labeled Nutrient agar plates. Use a sterile, disposable spreader to evenly distribute the diluted soil extract all over each culture plate. Repeat for the other two appropriate dilutions. Incubate all the plates at RT until visible colonies have appeared. Check the cultures after 24 hours to make sure they aren't close to being overgrown and again at 72 hours. Move the culture plates to the cold room before they become overgrown. Continue to check your plates every few days until you transfer them to the cold room. Your goal is to have between 30-300 countable colonies on one of the dilutions. <BR> | Using your P200 micropipet and sterile tips, dispense 100µl of the appropriate soil extract dilution onto each of two pre-labeled Nutrient agar plates. Use a sterile, disposable spreader to evenly distribute the diluted soil extract all over each culture plate. Repeat for the other two appropriate dilutions. Incubate all the plates at RT until visible colonies have appeared. Check the cultures after 24 hours to make sure they aren't close to being overgrown and again at 72 hours. Move the culture plates to the cold room before they become overgrown. Continue to check your plates every few days until you transfer them to the cold room. Your goal is to have between 30-300 countable colonies on one of the dilutions. <BR> | ||
2. '''Starch Medium (2.5%(wt/vol) soluble starch in Nutrient Agar) is used to determine the % of amalyase producing, starch digesting culturable microbes when compared to the total number counted in NA:'''<BR> | 2. '''Starch Medium (2.5%(wt/vol) soluble starch in Nutrient Agar) is used to determine the % of amalyase producing, starch digesting culturable microbes when compared to the total number counted in NA:'''<BR> | ||
