3,811
edits
BISC209/F13: Lab3: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
→Make another Soil Extract and Serial Dilution
| Line 40: | Line 40: | ||
<LI> Bottle of sterile water </ul><BR> | <LI> Bottle of sterile water </ul><BR> | ||
1. Label four large 18 mm sterile tubes 10<sup>-3</sup>, 10<sup>-4</sup>, 10<sup>-5</sup>, 10<sup>-6</sup> until you reach the proper dilution series for your soil extract | 1. Label four large 18 mm sterile tubes 10<sup>-3</sup>, 10<sup>-4</sup>, 10<sup>-5</sup>, 10<sup>-6</sup> until you reach the proper dilution series for your soil extract.<BR> | ||
2. Pipet 9 ml of sterile water into each tube using a 10 ml sterile, disposable serologic pipet. (You may use the same sterile 10 ml pipet for all of the tubes if asepsis is maintained.) <BR> | 2. Pipet 9 ml of sterile water into each tube using a 10 ml sterile, disposable serologic pipet. (You may use the same sterile 10 ml pipet for all of the tubes if asepsis is maintained.) <BR> | ||
3. Using a different sterile, disposable 1ml serologic pipet for each transfer, transfer 1ml of the 1% soil extract (1:100 dilution made of 1gram of soil) to the next dilution tube labeled 10<sup>-3</sup>; mix well by vortexing.<BR> | 3. Using a different sterile, disposable 1ml serologic pipet for each transfer, transfer 1ml of the 1% soil extract (1:100 dilution made of 1gram of soil) to the next dilution tube labeled 10<sup>-3</sup>; mix well by vortexing.<BR> | ||
