BISC209/F13: Lab3: Difference between revisions

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'''DATA COLLECTION'''<BR>
'''DATA COLLECTION'''<BR>
We have provided you with a spreadsheet called '''BIOLOG-CMD_calc&GraphTEMPLATE_2013_Tues_siteA (or B, or C, and _Wed_siteD, or E, or F)'''  You will find this spreadsheet on a thumb drive.  Please follow your instructor's adivice about how to insert your daily data after each reading into this template. You will have to carefully copy and paste the day's absorbance readings to the template. Be sure to use the correct one for your team! It is crucially important that you align the right carbon source to its value (don't forget water) and get the right well numbers transferred to the appropriate position on the Worksheet DAY (1, 2,etc) in the template workbook. Note that the Template contains a separate worksheet (tabs on the bottom) for each day within the full workbook. Make sure that you fill out the appropriate day (DAY 0, 1, 2 etc). If you miss a day, leave that page blank.  
We have provided you with a Google spreadsheet called '''BIOLOG-CMD_calc&GraphTEMPLATE_2013_Tues_siteA or B, and _Wed_siteD, or E, or F)'''  You will find this spreadsheet on a thumb drive.  Please follow your instructor's adivice about how to insert your daily data after each reading into this template. You will have to carefully copy and paste the day's absorbance readings to the template. Be sure to use the correct one for your team! It is crucially important that you align the right carbon source to its value (don't forget water) and get the right well numbers transferred to the appropriate position on the Worksheet DAY (1, 2,etc) in the template workbook. Note that the Template contains a separate worksheet (tabs on the bottom) for each day within the full workbook. Make sure that you fill out the appropriate day (DAY 0, 1, 2 etc). If you miss a day, leave that page blank.  


The Google Doc is pre-formatted with the calculations for these data. It includes the formulas to average replicate measurements each day and it will automatically subtract the background (readings in the water wells). There is a normalization for background that will be subtracted automatically (this threshold absorbance is 0.25 for each carbon source). We hope that including these pre-made calculations in the template will make your calculation of community metabolic diversity (CMD) and the data analysis of carbon source utilization patterns relatively uncomplicated. Please make sure you understand the EXCEL performed calculations. When you are sure that your data has been copied correctly into the appropriate template day, you will notice that the built in calculations will provide a final average absorbance reading for each substrate.   
The Google Spreadsheet is pre-formatted with the calculations for these data. It includes the formulas to average replicate measurements each day and it will automatically subtract the background (readings in the water wells). There is a normalization for background that will be subtracted automatically (this threshold absorbance is 0.25 for each carbon source). We hope that including these pre-made calculations in the template will make your calculation of community metabolic diversity (CMD) and the data analysis of carbon source utilization patterns relatively uncomplicated. Please make sure you understand the EXCEL performed calculations. When you are sure that your data has been copied correctly into the appropriate template day, you will notice that the built in calculations will provide a final average absorbance reading for each substrate.   


Fill in the last column labled: '''SCORE 1 for wells with OD above 0.0'''.  Once you fill in this final column, the template will calculate the total number of positive wells out of 31 for you and the SUM will appear in a cell below your data. This is the CMD for that day.  The total will also appear on the last tab of the workbook (CMDtable&graph).
Fill in the last column labled: '''SCORE 1 for wells with OD above 0.0'''.  Once you fill in this final column, the template will calculate the total number of positive wells out of 31 for you and the SUM will appear in a cell below your data. This is the CMD for that day.  The total will also appear on the last tab of the workbook (CMDtable&graph).
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