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BISC209/F13: Lab3: Difference between revisions
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→Make another Soil Extract and Serial Dilution
Tucker Crum (talk | contribs) |
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# Pour all of the suspension back into the flask and add a sterile magnetic stir bar (on your bench). | # Pour all of the suspension back into the flask and add a sterile magnetic stir bar (on your bench). | ||
#Place the flask on a magnetic stirrer at medium speed and mix for at least 15 min. | #Place the flask on a magnetic stirrer at medium speed and mix for at least 15 min. | ||
#Stop the stirring and let the soil settle until the larger particulate matter settles to the bottom. Not all visible particles need to settle, just the big stuff. | #Stop the stirring and let the soil settle until the larger particulate matter settles to the bottom. Not all visible particles need to settle, just the big stuff. When you take an aliquot from it, avoid the particulate matter in the lower portion. | ||
#In making this extract, you have created a 10<sup>-2</sup> or 1/100 dilution. This extract can also be described as a 1% (wt/vol) solution since there is 1 gram/100 ml. <BR><BR> | |||
'''Serial dilutions: '''(Each pair should make a dilution series using the same soil extract for the site).<BR> | '''Serial dilutions: '''(Each pair should make a dilution series using the same soil extract for the site).<BR> | ||
