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BISC209/F13: Lab2: Difference between revisions
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2. '''Goal for Cultured Bacteria Isolation: Obtain pure cultures of a variety of interesting bacteria in a soil community'''<BR> | 2. '''Goal for Cultured Bacteria Isolation: Obtain pure cultures of a variety of interesting bacteria in a soil community'''<BR> | ||
One goal in this project is for your group to end up with pure cultures of 2 unique soil bacteria per student. Work with your partners over the next several weeks so that you each choose different looking bacterial colonies to isolate to pure culture. | One goal in this project is for your group to end up with pure cultures of 2 unique soil bacteria per student. Work with your partners over the next several weeks so that you each choose different looking bacterial colonies to isolate to pure culture. Your goal is for each team member's subset to be unique and for the whole group sample to be as diverse as possible.<BR><BR> | ||
Note that isolation of desired bacteria from mixed culture is challenging. Your best technical and organizational skills are required. You will be expected to come in outside of lab time, on your own, to start, continue or complete the process of isolating to pure culture. We will make every attempt to make the media and reagents that you require available when you need them, but that availability requires advance planning on your part as well as on ours. Communicating your needs or desires well in advance of time of use, will reduce frustration and speed up the process. Remember that because this is an investigative, project based lab course, your instructors do not know the identity of the bacteria you are culturing from your soil habitat. The success of this project is in your hands. | Note that isolation of desired bacteria from mixed culture is challenging. Your best technical and organizational skills are required. You will be expected to come in outside of lab time, on your own, to start, continue or complete the process of isolating to pure culture. We will make every attempt to make the media and reagents that you require available when you need them, but that availability requires advance planning on your part as well as on ours. Communicating your needs or desires well in advance of time of use, will reduce frustration and speed up the process. Remember that because this is an investigative, project based lab course, your instructors do not know the identity of the bacteria you are culturing from your soil habitat. The success of this project is in your hands. | ||
