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BISC209/F13: Lab2: Difference between revisions
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→RICHNESS OF A MICROBIAL SOIL COMMUNITY: Culturing a few Bacteria from a Soil Community to examine structural diversity
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<LI>Your instructor will demonstrate this technique. Practice it carefully and you will not contaminate your plates with organisms found on your hands. <BR> | <LI>Your instructor will demonstrate this technique. Practice it carefully and you will not contaminate your plates with organisms found on your hands. <BR> | ||
<Li>Label the recipient plate on the bottom (NOT the lid!). | <Li>Label the recipient plate on the bottom (NOT the lid!). | ||
<LI>Flame your loop, let it cool a few seconds. | |||
<LI>Remove or tilt the lid of the donor agar plate with your non-dominant hand but keep the lid in your hand (don't put the lid down on the bench!). With your dominant hand holding the flamed and cooled loop, touch the loop to an interesting colony of bacteria that you want to study and pick up a TINY amount of the colony. Avoid touching other bacterial colonies. | |||
<LI>Close the lid of the donor plate. | |||
<Li>TIlt the lid of a new sterile plate of solid medium (in this case it will be Nutrient Agar) with your non-dominant hand so that it is partially open. Do not put the lid down on the bench; keep it in your hand! Touch the loop with the bacteria to the surface of the agar in an area near the periphery of the plate and gently glide the loop over the innoculum to spread it over a small area of the plate called Section 1 as shown in the illustration below. Do not gouge the agar! Replace the lid. | <Li>TIlt the lid of a new sterile plate of solid medium (in this case it will be Nutrient Agar) with your non-dominant hand so that it is partially open. Do not put the lid down on the bench; keep it in your hand! Touch the loop with the bacteria to the surface of the agar in an area near the periphery of the plate and gently glide the loop over the innoculum to spread it over a small area of the plate called Section 1 as shown in the illustration below. Do not gouge the agar! Replace the lid. | ||
<LI>Flame your loop and let it cool for a few seconds. | <LI>Flame your loop and let it cool for a few seconds. | ||
