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BISC209/F13: Lab2: Difference between revisions
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→Abundance Test 2: Enumeration of Community Soil Microorganisms by Direct Count of Microbial DNA Stained & Viewed by Fluorescence Microscopy
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Adapted from Schallenberg, M., Jacob, F. and Joseph B. R. (1989) Solutions to Problems in Enumerating Sediment Bacteria by direct counts. Applied & Environmental Microbiology. p. 1214-1219.<BR><BR> | Adapted from Schallenberg, M., Jacob, F. and Joseph B. R. (1989) Solutions to Problems in Enumerating Sediment Bacteria by direct counts. Applied & Environmental Microbiology. p. 1214-1219.<BR><BR> | ||
==Compare and Contrast== | |||
Compare this number to the calculation of CFUs/gram of wet soil (a culture-dependent assessment) that you will also obtain today. Compare the two counts that, in theory, should be the same since we are using two methods to figure out the same thing: how many microbes per gram comprise your soil community. The answer will provide evidence for one of our investigative goals: abundance or microorganisms in your soil community. What does it mean if your two experimental methods don't give the same answer? | |||
=='''RICHNESS OF A MICROBIAL SOIL COMMUNITY: <BR>Structural Diversity in Cultured Bacteria from a Soil Community'''== | =='''RICHNESS OF A MICROBIAL SOIL COMMUNITY: <BR>Structural Diversity in Cultured Bacteria from a Soil Community'''== | ||
