BISC209/F13: Lab2: Difference between revisions

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Directions for [[BISC209/F13: Streaking for Isolation| Streaking for Isolation]] are found in the [[BISC209/F13:Protocols| Protocols]] section of this wiki. <BR>
Directions for [[BISC209/F13: Streaking for Isolation| Streaking for Isolation]] are found in the [[BISC209/F13:Protocols| Protocols]] section of this wiki. <BR>


We hope that you have thousands of bacterial colonies on the NA+ starch and dilute NA plates that you set up last week for your colony enumeration. You and your partners should share these plates to find interesting bacteria that you would like to study. Your goal is that for student to end up with two pure cultures of bacteria that are different from your classmates'. Look for colonies that are different in color, texture, size, or other characteristics. We want to avoid isolating fungi so avoid black, or fuzzy growth that looks like mold. Check with your instructor after you and your partners have each selected a few colonies to attempt to isolate. You can circle the colony and put your initials on the plastic bottom on the plate to indicate the colonies you've chosen. When you are ready to sub-culture them, get new NA plates from the supply area (one per colony) and follow the directions for streaking for isolation described below.
We hope that you have thousands of bacterial colonies on the NA+ starch and dilute NA plates that you set up last week for your colony enumeration. You and your partners should share these plates to find interesting bacteria that you would like to study. Your goal is that for each student to end up with two pure cultures of bacteria that are different from your classmates. Look for colonies that are different in color, texture, size, or other characteristics. We want to avoid isolating fungi so avoid black, or fuzzy growth that looks like mold. Check with your instructor after you and your partners have each selected a few colonies to attempt to isolate. You can circle the colony and put your initials on the plastic bottom on the plate to indicate the colonies you've chosen. When you are ready to sub-culture them, get new NA plates from the supply area (one per colony) and follow the directions for streaking for isolation described below.


'''Streaking for Isolation from Solid Medium to Solid Medium:'''<BR>
'''Streaking for Isolation from Solid Medium to Solid Medium:'''<BR>
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