BISC209/F13: Lab2: Difference between revisions

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<li>Repeat to streak sections 3 and 4 by dragging your loop through the last section innoculated once or twice and then to a new area of the plate. Replace the lid.
<li>Repeat to streak sections 3 and 4 by dragging your loop through the last section innoculated once or twice and then to a new area of the plate. Replace the lid.
<li>Label the recipient plate on the bottom (NOT the lid!), invert it, and incubate the plate at RT.
<li>Label the recipient plate on the bottom (NOT the lid!), invert it, and incubate the plate at RT.
<li>Check your plate for colonies daily and record your descriptions of the texture and shape of the colonies that appear.  If any colonies arise that look tough and leathery or appear as "little, powdered-sugar volcanos", use the tip of a sterile toothpick to pick up a small but visible amount of growth (being careful not to touch anything but the tip of the colony) and "spread" the bacterial growth onto section 1 of a new nutrient agar (NA) plate. Only inoculate one colony/plate and streak for isolation into 4 sections as described above.
<li>Check your plate for colonies daily and record your descriptions of the texture and shape of the colonies that appear.   
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This process of transferring a single well isolated colony to new solid medium will continue your attempt until you succeed in isolating two different bacteria to pure culture. Our goal as a course is to find a diverse group of interesting soil bacteria . <BR><BR>
This process of transferring a single well isolated colony to new solid medium will continue your attempt until you succeed in isolating two different bacteria to pure culture. Our goal as a course is to find a diverse group of interesting soil bacteria . <BR><BR>
The directions for [[BISC209/F13: Aseptic Transfer| Aseptic transfer: Broth to Broth and Broth to Plate]] are found in the [[BISC209/F13:Protocols| Protocols]] section of the wiki. <BR>


==CLEAN UP==
==CLEAN UP==
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