BISC209/F13: Lab2: Difference between revisions

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BISC209/F13: Lab2 (view source)

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→‎RICHNESS OF A MICROBIAL SOIL COMMUNITY: METABOLIC DIVERSITY: Structural Diversity in Cultured Bacteria from a Soil Community
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<font color="purple">'''Enrichment & Isolation of endospore-forming bacteria from oven dried soil extract on Glycerol Yeast Extract (GYEA) Medium:'''</font color="purple">
0.5% (v/v) Glycerol, 0.2% Yeast Extract, 0.1%K<sub>2</sub>HPO<sub>4</sub>(Dipotassium phosphate), 1.5% Agar''' <BR><BR>
Endospore forming genera include ''Bacillus'' and ''Streptomyces'' as well as other members of the ''Actinomycetes'' family''. Many of these bacteria are antibiotic producers.<BR><BR>
Oven drying will have killed most of the vegetative non-spore forming microbes. GYEA medium is particularly selective for the ''Streptomyces'' group of endospore formers because of the osmotic pressure obtained from the high concentration of glycerol. Few bacteria can thrive in GYEA which favors the growth of members of the Streptomyces group of Gram positive bacteria. <BR>
'''In pairs''' set up enrichment/selection cultures for spore-forming bacteria on two plates of glycerol yeast extract agar (GYEA) following the directions below (also found in the [[BISC209/F13: Culture Media| Enrichment Media for the Isolation of Soil Bacteria in a Mixed Population: Finding Spore Forming Bacteria]] in the Protocols: Culture Media section of this wiki).
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'''Isolation of Spore-formers by isolation streak:'''<BR>
'''Isolation of Bacteria by isolation streak:'''<BR>
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<Li>You will use a new sterile cotton swab dipped into the oven-dried-soil extract to Swab section 1 of a pre-labeled plate of glycerol yeast medium (GYEA).
<Li>You will use a new sterile cotton swab dipped into the oven-dried-soil extract to Swab section 1 of a pre-labeled plate of NA medium .
<LI>Wait one minute to allow the inoculum in section 1 to absorb into the agar before you switch to your inoculating loop to streak from section 1 into section 2, following the steps for [[BISC209/F13: Streaking for Isolation| Streaking for Isolation]] in the [[BISC209/F13:Protocols| Protocols]] section of this wiki.  
<LI>Wait one minute to allow the inoculum in section 1 to absorb into the agar before you switch to your inoculating loop to streak from section 1 into section 2, following the steps for [[BISC209/F13: Streaking for Isolation| Streaking for Isolation]] in the [[BISC209/F13:Protocols| Protocols]] section of this wiki.  
<LI>Flame your loop and isolation streak section 2.
<LI>Flame your loop and isolation streak section 2.
Tucker Crum
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