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BISC209/F13: Lab2: Difference between revisions
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→LAB 2: Enumerating Microbial Members & Isolating Bacterial Members of a Soil Community
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==LAB 2: Enumerating Microbial Members & Isolating Bacterial Members of a Soil Community== | ==LAB 2: Enumerating Microbial Members & Isolating Bacterial Members of a Soil Community== | ||
Today you will complete your first investigative goal in our semester long project: assessing abundance of a soil microbial community by enumerating its microbes by two methods, one culture dependent and one culture independent. Today you will also continue acquiring and practicing skills used by working microbiologists. In addition, you will continue | Today you will complete your first investigative goal in our semester long project: assessing abundance of a soil microbial community by enumerating its microbes by two methods, one culture dependent and one culture independent. Today you will also continue acquiring and practicing skills used by working microbiologists. You will complete your tests for abundance today. The results of the fluorescent DNA stain are available and your plate counts are ready to assess. In addition, you will continue to isolate a few bacteria of interest from your soil community in order to show evidence for richness (diversity) and potential for co-operative and competitive behaviors among community members. Next week we will begin testing the whole soil microbial community : a culture-dependent assessment of carbon source utilization diversity. | ||
==Finishing the Standard Plate Count of Culturable Soil Microbial Community== | ==Finishing the Standard Plate Count of Culturable Soil Microbial Community== | ||
