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Chromosomal DNA isolation from E. coli: Difference between revisions
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Chromosomal DNA isolation from E. coli (view source)
Revision as of 08:16, 31 July 2012
, 31 July 2012→Procedure
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* incubate 5 min at 65°C | * incubate 5 min at 65°C | ||
* add 750 μL isopropanol and mix | * add 750 μL isopropanol and mix | ||
* spin at max. speed for 5 min | * spin at max. speed for 5 min at room temperature and discard supernatant | ||
* resuspend pellet in 500 μL TE and add 2 μL RNase A (25 mg/ml) | * resuspend pellet in 500 μL TE and add 2 μL RNase A (25 mg/ml) | ||
* incubate for 30 min at 65°C | * incubate for 30 min at 65°C | ||
