Proteinase K

Purpose

• Proteinase K is used to degrade proteins in DNA purification or ChIP-Chip experiments.

Procurement

• Proteinase K can be bought at Qiagen or Fermentas

Use

• Usual stock solution is 20mg/ml in for example TBS
  • Stock solution can be stored at -20°C
• Optimal temperature is 50 to 55 °C
• Can be denatured at 95 °C for 10 min
• The recommended working concentration for Proteinase K is 0.05-1 mg/ml.

References

Fermentas data sheed