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BioBuilding: Synthetic Biology for Teachers: Lab 1: Difference between revisions
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BioBuilding: Synthetic Biology for Teachers: Lab 1 (view source)
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This lab offers two different protocols based on the time the teacher wishes to allow. Each of these protocols covers the same concepts but allows for different emphases. | This lab offers two different protocols based on the time the teacher wishes to allow. Each of these protocols covers the same concepts but allows for different emphases. | ||
Protocol A: This is a shorter procedure for the students. This shorter protocol allows for more emphasis on microbiological techniques. This protocol is recommended should the teacher choose to have the students do the initial bacterial culturing. Essentially, a day prior to any data collection, the large cultures are set up. Instead of letting them all run, part of the culture is immediately removed and placed in the refrigerator. This will be the lag phase sample. After 5-7 hours, a second sample is removed. This will be the log phase sample. The last third of the culture will be allowed to grow overnight. This will be the stationary phase sample. This will allow the students to easily obtain data in one lab period. | Protocol A: This is a shorter procedure for the students. This shorter protocol allows for more emphasis on microbiological techniques. This protocol is recommended should the teacher choose to have the students do the initial bacterial culturing. Essentially, a day prior to any data collection, the large cultures are set up. Instead of letting them all run, part of the culture is immediately removed and placed in the refrigerator. This will be the lag phase sample. After 5-7 hours, a second sample is removed. This will be the log phase sample. The last third of the culture will be allowed to grow overnight. This will be the stationary phase sample. This will allow the students to easily obtain data in one lab period. | ||
If time allows the students can carry out all parts of the procedure. This will take about 5 class periods in a typical High School AP Biology or Biotechnology class. If instead the teacher prepares in advance the samples as outlined in Part 1 of the procedure, the students can conduct the smell tests and population measurements over two or three days. In a college lab course, most of the bacterial growth curve can be conducted in a typical three to four hour lab period. The procedure includes instructions for using a spectrophotometer to measure the population growth. If a spectrophotometer is not available, the population can be easily measured using the McFarland Turbidity methodology, as explained below. | If time allows the students can carry out all parts of the procedure. This will take about 5 class periods in a typical High School AP Biology or Biotechnology class. If instead the teacher prepares in advance the samples as outlined in Part 1 of the procedure, the students can conduct the smell tests and population measurements over two or three days. In a college lab course, most of the bacterial growth curve can be conducted in a typical three to four hour lab period. The procedure includes instructions for using a spectrophotometer to measure the population growth. If a spectrophotometer is not available, the population can be easily measured using the McFarland Turbidity methodology, as explained below. | ||
