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BioMicroCenter:Caliper LabChip GX: Difference between revisions
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8. The Total RNA concentration in the sample is computed from the ratio of the RNA area in the sample to the RNA area in the ladder multiplied by the ladder concentration specified in the assay. | 8. The Total RNA concentration in the sample is computed from the ratio of the RNA area in the sample to the RNA area in the ladder multiplied by the ladder concentration specified in the assay. | ||
9. Assay-defined RNA fragments are identified from the peaks in the peak table. Fragments | 9. Assay-defined RNA fragments are identified from the peaks in the peak table. Fragments are located by finding the largest peak within a size range associated with the fragment.For Eukaryote RNA assays, 5S, 18S and 28S fragments are located. For Prokaryote assays, 5S, 16S and 23S fragments are identified. | ||
are located by finding the largest peak within a size range associated with the fragment. | |||
For Eukaryote RNA assays, 5S, 18S and 28S fragments are located. For Prokaryote | [[Image:BioMicroCenter_caliper RNA.jpg|center|200px]] | ||
assays, 5S, 16S and 23S fragments are identified. | |||
10. Messenger RNA Assay: The RNA contamination ratio is computed. This is the ratio of the area of all the fragments to total RNA area. | |||
=== [[BioMicroCenter:Caliper_INFO | Software]] === | === [[BioMicroCenter:Caliper_INFO | Software]] === | ||
The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | The LabChip GX software for data analysis allows users to visualize results via an electropherogram or virtual gel view and provides data in tabular form, which can then be analyzed or easily exported into a spreadsheet format. The soft ware is available for download from BioMicro at http://biomicro-bioanalyzer.mit.edu/ | ||
