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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 18:24, 30 September 2010
, 30 September 2010→BISC219 F10 Weekly Lab Planner
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| '''Series2:Mapping:'''Self double mutants to keep viable true-breeding progeny;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | | '''Series2:Mapping:'''Self double mutants to keep viable true-breeding progeny;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it was used to id | | '''Homework:''' Explain complementation analysis in general and specifically how it was used to id your ''dpy'' gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | ||
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! 6 | ! 6 | ||
