BISC 219/F10:Gene Mapping

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Background: Classical Forward Genetics and Gene Mapping
Lab 2: Mutant Hunt
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2

Lab # Dates Activity Outside Activity
2 9/14 - 9/20 Mutant Hunt Confirm mutant phenotype and set up first cross
3 9/21 - 9/27 Linkage crosses - heterozygous males with Unc hermaphrodites Pick hermaphrodites for selfing
4 9/28 - 10/4 Examine phenotypes to determine linkage

Mapping: Pick Unc non-Dpy to separate plates;
Complementation: cross unknown Dpy worms to wild type males

Mapping: Pick putative double mutants
Complementation: cross heterozygous males with known Dpy mutants
5 10/5 - 10/12 Mapping: Maintain double mutants (d u/d u): pick a few to individual plates
Complementation: examine crosses for Dpy MALES
6 10/13 - 10/19 DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product;
Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)
Bioinformatics:Use Wormbase and BLAST to learn more about the gene in other species;
Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
7 10/20 - 10/26 Complete Mapping

SCORE! Find recombinant frequency

Determine map distance between your Dpy and Unc marker