BISC 219/F10: Assignment Series2 Outline Summary

From OpenWetWare
Jump to navigationJump to search

Assignment Series2 Outline & Summary

Please construct a complete yet concise statement of the overall topic of investigation in Series 2 and the experimental question & goals of our classical (forward) genetics approach to understanding the structural and functional relationship between a gene and the product it encodes. Outline the entire experimental approach to achieving these experimental goals, including all of the following parts to our project: screening for a mutant, linkage analysis, mapping the mutation's location to a chromosome and gene, complementation analysis and what it will tell you, defining the gene sequence change responsible for the gene product change that is associated with the functional defect through DNA sequence analysis and comparison with wild type gene sequence, and bioinformatics using Wormbase or other BLAST databases to determine significance of the mutation in C. elegans or in other species through a search for homologues. This outline should NOT be a detailed list of exactly what you DO in lab each week but should, instead, be a brief summary of the conceptual goals of each part of the work that is part of our project. After you have outlined the experimental work in this project, please use that outline to construct a 1-2 page narrative summary in prose of how you will determine the exact location of the gene, the sequence change that is likely to be responsible for the phenotypic anomaly you found initially, and the significance of the change in C. elegans and in other species.

Grading Rubric

Assignment Lab 2– 10 points

At or Above Standard Below Standard Possible


& Experimental Question
Constructed a clear, concise, and accurate description of the overall topic, the experimental question(s) and goals of the Series 2 investigation Topic or experimental question(s)/goals missing, inaccurate, partially accurate; contains too much tangential information or is too simplified 2 __/2
of Experimental Approach in Series 2: Classical or Forward Genetics
Constructed a complete, clear, concise, and accurate outline or flow diagram of ALL parts of Series2 including: mutant screen, linkage testing, mapping , complementation testing, determining if new mutation or previously characterized, DNA sequencing analysis, WormBase data base work Outline/diagram is missing elements, is inaccurate or partially inaccurate; contains too much tangential information or is too simplified. 4 __/4
Narrative summary of project
Constructed a clear, concise, accurate, and logically developed description of the experimental approach to locating and characterizing the gene and gene mutation responsible for a phenotypic alteration from wild type Experimental analysis missing, inaccurate, partially accurate; contains too much tangential information or is too simplified, isn't logically developed 4 __/4
Total 10 __/10