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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 17:52, 30 September 2010
, 30 September 2010→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
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! 5 | ! 5 | ||
| Oct. 5 to<br> Oct. 12 | | Oct. 5 to<br> Oct. 12 | ||
| ''' | | '''Series2:Mapping:Self double mutants to keep viable true-breeding progeny<BR>'''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross. Cross these wild type males (+ +/+ +) X your double mutant (d u/d u). | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross. Cross these wild type males (+ +/+ +) X your double mutant (d u/d u). | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it was used to id, potentially anyway, your ''dpy'' gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | | '''Homework:''' Explain complementation analysis in general and specifically how it was used to id, potentially anyway, your ''dpy'' gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | ||
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! 6 | ! 6 | ||
| Oct. 13 to <br> Oct. 19 | | Oct. 13 to <br> Oct. 19 | ||
| ''' | | '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);Self double mutants to new plate<BR> Series2: Characterizing the dpy mutation''' Gene Sequence Analysis <br> '''Series3: Reverse Genetics:'''Restriction enzyme digest PCR product/clean-up/ligation into pPD129.36 vector/transformation into BL21 ''E.coli'' | ||
| | | Series 2:Cross heterozygous (++/dyp unc) males with L4 double mutant hermaphrodites.<BR>Series 3:Check control & transformation plates (save) – <BR>notify instructor if no colonies | ||
| '''Homework''': DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at [[BISC_219/F10: Assignment_Series2_DNA Sequencing]]<BR> | | '''Homework''': DNA sequencing Analysis due at the beginning of Lab 7. Assignment description at [[BISC_219/F10: Assignment_Series2_DNA Sequencing]]<BR> | ||
Scientific research report on Series2 Classical (Forward) Genetics Project due week of 11/1 at the beginning of lab. 5%/day late penalty! See assignment directions at: [[BISC 219/F10: Assignment_ Series2_Classical Genetics Paper]] | Scientific research report on Series2 Classical (Forward) Genetics Project due week of 11/1 at the beginning of lab. 5%/day late penalty! See assignment directions at: [[BISC 219/F10: Assignment_ Series2_Classical Genetics Paper]] | ||
| Line 119: | Line 119: | ||
! 7 | ! 7 | ||
| Oct. 20 to <br> Oct. 26 | | Oct. 20 to <br> Oct. 26 | ||
| '''Series3:Reverse Genetics''' Colony PCR to look for bacterial transformants | | '''Series2:Finish Mapping:''' SCORE! Calculate Recombination frequency; <BR>'''Series3:Reverse Genetics''' Colony PCR to look for bacterial transformants | ||
| '''Day before lab:''' grow overnight culture of positive colony | | Determine map distance between your mutant gene <BR>and the known reference mutation'''Day before lab:''' grow overnight culture of positive colony | ||
| '''Homework''': | | '''Homework''': Work on your Series 2: Forward genetics paper due week of Nov 1 | ||
|- | |- | ||
! 8 | ! 8 | ||
