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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 13:32, 27 September 2010
, 27 September 2010→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
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| '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; | | '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross. Cross these wild type males (+ +/+ +) X your double mutant (d u/d u). | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross. Cross these wild type males (+ +/+ +) X your double mutant (d u/d u). | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it was used to potentially | | '''Homework:''' Explain complementation analysis in general and specifically how it was used to id, potentially anyway, your ''dpy'' gene and to determine if the mutation of interest has been previously characterized. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | ||
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! 6 | ! 6 | ||
