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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 13:29, 27 September 2010
, 27 September 2010→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
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| '''Complete Linkage Analysis:''' examine phenotypes and count to determine linkage;<BR> '''Part 3: Start Mapping:''' pick (6) Uncs of the linked unc''' strain to separate plates (6 plates total); '''Part 4: Start Complementation Analysis:''' Cross Dpy mutant worms to N2 males (2 plates total) | | '''Complete Linkage Analysis:''' examine phenotypes and count to determine linkage;<BR> '''Part 3: Start Mapping:''' pick (6) Uncs of the linked unc''' strain to separate plates (6 plates total);<BR> '''Part 4: Start Complementation Analysis:''' Cross Dpy mutant worms to N2 males (2 plates total) | ||
| '''3 days after lab: Mapping:''' <BR>Pick (3) double mutants to separate plates (3 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR> | | '''3 days after lab: Mapping:''' <BR>Pick (3) double mutants to separate plates (3 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR> | ||
| '''Homework:''' Draw crosses and diagram strategy for Mapping the Location of your ''dpy'' mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 5. Assignment described at [[BISC_219/F10: Assignment_Series2_Mapping Crosses]]; Read the journal article by Davis ''at al.'',Rapid single nucleotide polymorphism mapping in C. elegans, found at BMC Genomics 2005, 6:118 [http://dx.doi.org/10.1186/1471-2164-6-118 doi:10.1186/1471-2164-6-118] and compare their mapping strategy to yours. | | '''Homework:''' Draw crosses and diagram strategy for Mapping the Location of your ''dpy'' mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 5. Assignment described at [[BISC_219/F10: Assignment_Series2_Mapping Crosses]]; Read the journal article by Davis ''at al.'',Rapid single nucleotide polymorphism mapping in C. elegans, found at BMC Genomics 2005, 6:118 [http://dx.doi.org/10.1186/1471-2164-6-118 doi:10.1186/1471-2164-6-118] and compare their mapping strategy to yours. | ||
