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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 13:28, 27 September 2010
, 27 September 2010→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
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! 4 | ! 4 | ||
| Sept. 28 to <br> Oct. 4 | | Sept. 28 to <br> Oct. 4 | ||
| '''Complete Linkage Analysis:''' examine phenotypes and count to determine linkage;<BR> '''Part 3: Start Mapping:''' pick ( | | '''Complete Linkage Analysis:''' examine phenotypes and count to determine linkage;<BR> '''Part 3: Start Mapping:''' pick (6) Uncs of the linked unc'' strain to separate plates (6 plates total); '''Part 4: Start Complementation Analysis:''' Cross Dpy mutant worms to N2 males (2 plates total) | ||
| '''3 days after lab: Mapping:''' <BR>Pick (3) double mutants to separate plates (3 plates total);<BR> | | '''3 days after lab: Mapping:''' <BR>Pick (3) double mutants to separate plates (3 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR> | ||
''' | |||
| '''Homework:''' Draw crosses and diagram strategy for Mapping the Location of your ''dpy'' mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 5. Assignment described at [[BISC_219/F10: Assignment_Series2_Mapping Crosses]]; Read the journal article by Davis ''at al.'',Rapid single nucleotide polymorphism mapping in C. elegans, found at BMC Genomics 2005, 6:118 [http://dx.doi.org/10.1186/1471-2164-6-118 doi:10.1186/1471-2164-6-118] and compare their mapping strategy to yours. | | '''Homework:''' Draw crosses and diagram strategy for Mapping the Location of your ''dpy'' mutation. (Complete template downloaded for Linkage analysis). Due at the beginning of Lab 5. Assignment described at [[BISC_219/F10: Assignment_Series2_Mapping Crosses]]; Read the journal article by Davis ''at al.'',Rapid single nucleotide polymorphism mapping in C. elegans, found at BMC Genomics 2005, 6:118 [http://dx.doi.org/10.1186/1471-2164-6-118 doi:10.1186/1471-2164-6-118] and compare their mapping strategy to yours. | ||
|- | |- | ||
! 5 | ! 5 | ||
| Oct. 5 to<br> Oct. 12 | | Oct. 5 to<br> Oct. 12 | ||
| '''Series 2: Mapping''': cross N2 males with double mutants (2 | | '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene;<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross. Cross these wild type males (+ +/+ +) X your double mutant (d u/d u). | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross. Cross these wild type males (+ +/+ +) X your double mutant (d u/d u). | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your ''dpy'' mutation. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | | '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your ''dpy'' mutation. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | ||
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