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BISC 219/F10:Calendars/Planner: Difference between revisions
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BISC 219/F10:Calendars/Planner (view source)
Revision as of 08:37, 27 September 2010
, 27 September 2010→BISC219 F10 Weekly Lab Planner
Tucker Crum (talk | contribs) |
Tucker Crum (talk | contribs) |
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| Oct. 5 to<br> Oct. 12 | | Oct. 5 to<br> Oct. 12 | ||
| '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | | '''Series 2: Mapping''': cross N2 males with double mutants (2 plates total);<BR> '''Series2: Complementation:''' pick males from complementation cross #1 and mate with known Dpy strains (3 plates total);<BR> <BR> '''Series3: Reverse Genetics:''' Pick gene of interest; set up PCR reaction to clone the gene | ||
| '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR> | | '''Series 3:Reverse Genetics:''' Examine the results of agarose gel electrophoresis<BR>'''3 days after lab:''' '''Series2: Mapping:''' pick (4) <BR>heterozygotes from previous cross to individual plates (4 plates total);<BR>'''Series2:Complementation:''' examine cross plates for Dpy males - WHY?; | ||
| '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your ''dpy'' mutation. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | | '''Homework:''' Explain complementation analysis in general and specifically how it will be used to confirm and expand your characterization of your ''dpy'' mutation. Diagram complementation crosses. Template downloadable at: [[Media:Complementation_Template_Crosses.pptx]]Due at the beginning of Lab 6. Assignment described at[[BISC_219/F10: Assignment_Series2_Complementation]] | ||
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